FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254752
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9200.038.3799171
TCGCTCT10800.035.999764
TCTGTCG10550.031.3931588
GTCTTGC19300.030.989641
TCTCGCT11900.030.8583072
CTCGCTC12050.029.2778133
CGCTCTG11550.026.8050175
CCGTTCG1400.00104108725.7141154
GTATTCG1701.0754062E-425.4330271
GTATATA9250.024.928951
ATAATAC6400.024.7498383
GTATAGA7350.024.5102881
GAGTGCA49300.024.0972046
GTGTAAA13450.023.5736121
CTGTCGC15600.023.5377859
TAATACT7100.023.323794
GTATTGA7450.023.2140391
GTCGTAG2252.7811242E-522.4187431
GGAGTGC47350.022.3525435
GTGTAAC5850.022.1723841
Produced by FastQC (version 0.11.5)