FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454048
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11050.039.131351
TCGCTCT12050.037.642824
TCTGTCG12850.030.2565388
TCTCGCT13650.030.0675052
GTGTTAC9550.029.4304641
CTCGCTC13800.029.217133
CGCTCTG14000.028.2854615
GTCTTGC21250.028.1484851
GTATTAG7950.024.4755521
TATTAGA7100.023.3251612
GTATTAA6600.022.9303781
CTATTAG5400.022.687731
GTGTTAG16950.022.5341951
GCGCCGA3055.7151556E-721.2457146
CGTTTCG1700.003221308621.175859
GTTACCC8600.020.9300483
GTCTCAC27100.020.7425041
CTGTCGC19450.020.729479
GTACGGA1750.0037895120.5905441
TAAGACT9450.020.5712454
Produced by FastQC (version 0.11.5)