FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586412
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10000.037.4698451
TCGCTCT12950.033.358564
GTCTTGC18500.030.3809571
TCTCGCT13200.028.9092032
TCTGTCG13150.027.9235218
CTCGCTC13700.027.8535843
CGCTCTG14250.026.7785365
GTCTTAC7750.026.033641
GTCTAGC7750.025.1038681
GTATTAA5950.024.2209721
TCGAACT17400.023.9991669
GTACTAA4900.023.5289461
GGAGTGC54050.023.4445975
GAGTGCA59550.023.3346546
CTCGAAC18450.022.6337978
GTACCGG1600.002254905622.5179351
GTCTCAC23800.022.40441
CTGGAGT67400.022.3260653
TATCCCG1952.7419807E-422.1535035
GTCTCGA25750.022.088986
Produced by FastQC (version 0.11.5)