FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631472
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11050.045.6420061
TCTGTCG11150.044.5525938
CTGTCGC12900.039.066739
GTCTTGC24250.031.7907661
TCGCTCT12000.031.1975754
TCTCGCT13900.030.582022
TTATACT8200.027.2173984
CTACGCT1607.165641E-526.99794
CGCTCTG14300.026.683245
GTATTAG8650.026.6540281
GTCTCAC31250.026.2835351
CTCGCTC14900.025.1446763
GTATAAC4101.8189894E-1224.6021521
GTGTTAG19150.024.0790941
GTACTAC4550.023.7524721
GTTTCAC37100.023.6927173
GTGTTAC11600.023.6023121
TTTACGC1550.001876124523.2416653
GTACGCC1550.001876124523.2416653
GTATAGT7850.022.9455931
Produced by FastQC (version 0.11.5)