FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577168
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10750.042.225811
TCTGTCG9250.037.358678
TCGCTCT12750.034.4438364
GTCTTGC22850.031.8479231
CTGTCGC11850.031.5919829
TCTCGCT14200.031.4593142
GTATAAC6450.030.1612931
CTCGCTC13500.029.3545113
CGCTCTG14250.028.7973065
GGGTAAC10200.026.1365031
GTATTAA8600.024.2965971
TACACTG15400.023.8419275
CTTACGC3102.5862391E-823.2425733
GTATTAC6400.022.5162451
GGTATAC5800.022.3609581
TTGCTCT30100.022.0045874
GTATTAT9500.021.9948141
TTACACT12800.021.935454
GTCTTAC11500.021.9288641
TATACGG1650.002710092821.816145
Produced by FastQC (version 0.11.5)