FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311225
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9100.046.6760988
CTGTCGC10200.038.1133889
GTCTCGC10200.033.906441
GTCTTGC21700.033.203311
GACCGTC2553.263267E-928.232147
GTATAGA7700.028.071891
GTCTTAC10100.027.1083681
TCGCTCT10350.026.4318264
TCTCGCT12900.026.2512052
GGTATAC5000.025.9384251
TTGCTCT28900.025.9071394
TAATACT7050.024.5078984
GTATTAT8850.024.424131
GGACCGT3251.6134436E-924.3665056
GTGTATA13250.023.9264321
GTATTAG6700.023.6585981
GTATAAT7050.023.506061
GTGTAAC6750.023.4833491
CTCGCTC11500.023.1816843
GTATAAA8750.023.056381
Produced by FastQC (version 0.11.5)