FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610312
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10900.037.677411
TCTGTCG10550.035.48488
CTGTCGC11000.034.6876349
TCGCTCT10400.033.9198724
TCTCGCT12000.032.42242
CTCGCTC12700.029.50073
CGCTCTG13600.027.5268125
GTATTAC6100.025.9851681
GTACGGA2808.771167E-925.7320671
GTGTTAG19500.024.7555681
GTCTTGC24350.024.5590631
GTATAAG8850.023.6095331
GTATTAA8650.023.3224741
GTATATA13300.022.7525631
TACACCG1902.3012278E-422.7346955
GTCTCAC29500.022.4697631
GTGTAAA17000.022.462581
GTGTAAC7700.022.4570771
GTGTAGA13550.021.269311
GTGTTAC10850.021.2497041
Produced by FastQC (version 0.11.5)