FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610312
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG450.00990975947.9979139
TCTGTCG9900.032.7264758
TCGCTCT12800.031.5016484
GTCTCGC11250.031.3760641
GTCTTGC22200.030.1776121
GTGTTAC9700.028.2206361
CGCTCTG12500.027.6473265
CTGTCGC12350.027.3996189
CTCGCTC14850.026.1831863
GTACCGG2259.640935E-725.6131151
GGTATAC5100.025.4247841
CGTTTCG1701.0819188E-425.410669
GTATTAA8550.025.27611
TCTCGCT15100.024.7997992
GTACTAC5000.024.492541
GTATTAC6800.023.3060511
GTATAGG6800.023.3060511
GTGTAAA16700.023.2933641
TACACTG14850.023.272165
GTCTTAC9700.023.0220991
Produced by FastQC (version 0.11.5)