FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537488
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10500.039.1035081
TCTGTCG9500.037.8925638
TCCGTCG1301.7367594E-533.2288638
CTGTCGC11700.032.6135189
TCGCTCT11550.032.4137274
GTCTTGC21450.031.2309931
CTCGCTC11900.028.449923
GTCCGGT2705.9699232E-926.6788061
GTATAAC4100.026.3534561
TCTCGCT13950.026.3345622
GTCTCAC30650.024.4417921
GGAGTGC59000.024.1613255
TAATACT8700.023.9986234
GTATATA12700.023.8218631
GTGTTAG18200.023.3512821
GTCTTAC10250.023.191041
CGCTCTG13850.022.8723355
GTTTCAC39700.022.6803443
GTATAAT7000.022.6388721
GAGTGCA65550.022.5158716
Produced by FastQC (version 0.11.5)