FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537488
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.046.8666088
GTCTCGC9600.042.7732431
CTGTCGC13200.040.3625039
TCGCTCT12750.032.753964
TCTCGCT13000.031.5764332
CGCTCTG13400.027.9395165
GTCTTGC23300.027.5171011
CTCGCTC14150.027.4778843
GCGTAAT1358.392994E-426.6811641
TAACACG5450.025.1016984
GGTATAC5750.025.0570951
GTATTAC6250.024.2051541
GTCTAGC10450.024.1279451
GTACTAC5150.023.7799111
GTCTCAC28850.023.721731
GTGTAAC7700.023.3893341
GTTCTAC7500.023.0525261
GTATAAC4403.6379788E-1222.9215451
GTGTAAA15950.022.5828061
TAACACT12350.022.1545334
Produced by FastQC (version 0.11.5)