FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229256
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.045.778978
GTCTCGC10500.044.5900881
TCGCTCT11950.037.9570164
GTCTTGC20200.036.728241
TCTCGCT12550.035.5846142
CTGTCGC11550.035.5314039
CTCGCTC13200.034.3780253
TAGCCCG1102.538592E-432.726295
CGGTACT1102.538592E-432.726294
CGCTCTG13450.032.6532945
GTCTCAC24950.029.7358931
TTGCTCT27800.026.4164714
GTGTTAG15600.026.3187081
TAATACT6850.025.2255214
CTTGCTC26300.025.1968573
GTATAAT7150.025.1853681
GTGTTAC10500.024.6960491
GTATAAC4251.8189894E-1223.7275771
GTTAGCC12850.023.5429273
GTATTAC5250.023.3240471
Produced by FastQC (version 0.11.5)