FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229256
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8700.039.7244038
GTCTTGC19600.037.8607671
CTGTCGC10750.034.8273629
GTCTCGC9600.033.771441
GTCTTAC9700.031.1950571
TACGCTT1402.8810815E-530.8573515
TAATACT6300.028.571624
TCGCTCT10950.028.2741624
GTATTAT5700.027.8071211
GGTATAC5200.027.7098941
TTCACCG5550.025.946125
GTGTAAC7550.025.7646981
TCTCGCT12150.025.4890842
GTATTAC5400.025.3494241
CTCGCTC11700.025.230943
GTATTAG8000.025.2160031
GTCTCAC28350.024.142311
GTATATA9450.023.6340481
GTATAAC3753.3287506E-1023.0546341
GTGTAAG12850.022.9873541
Produced by FastQC (version 0.11.5)