FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095361
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9100.040.3506478
GTCTTGC19400.040.1054041
GTCTCGC8800.038.476541
CTGTCGC10750.038.1757979
TCGCTCT10050.034.3872034
GTATTAG9100.030.0831341
GGCGTAC1306.7305705E-427.7081491
TCTCGCT11300.027.4139022
GTATACG1856.1145383E-627.2588271
GTTCTAC5900.026.8628161
GTACTAT5150.026.5783021
CGCTCTG11600.026.0683175
CTTGCTC27200.025.691163
TTGCTCT28650.025.3815694
CTCGCTC12250.024.6998353
GTATAAC3801.4551915E-1124.645671
GTGTAAC6800.024.3668731
GTTACCC6400.023.6385143
TAATACG1851.9198666E-423.350774
GCGTATA1550.00187720623.2390921
Produced by FastQC (version 0.11.5)