FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946561
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12900.041.8849831
TCTGTCG12100.038.0790868
GTCTTGC27500.034.8422131
TCGCTCT15300.033.408644
TCTCGCT15850.032.725782
CTCGCTC16900.030.6925223
CTGTCGC15400.030.3867729
GTATAAC6200.029.0492631
GTGTTAC12800.027.5786441
CGCTCTG17150.026.4465085
GCGTAAT1400.001037572225.7293451
TTGCTCT35450.025.1824134
GTATAGG7300.024.6719761
GTATTAG9600.024.0140571
GTATAAG8200.023.7212031
GGTATAC6450.023.4555911
GTCTTAC12000.023.4137041
GTCTAAC5900.023.2000221
GTCTCAC35650.023.0373271
GCGTTAC1902.2907551E-422.750161
Produced by FastQC (version 0.11.5)