FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946561
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.043.1971978
TCGCTCT13800.038.609474
GTCTCGC13250.036.966741
CTGTCGC15850.035.429889
GTCTTGC24950.034.3553621
TCTCGCT15800.030.992172
GTCTTAC10500.030.8703351
CTCGCTC16750.030.5200143
GTATAGA8750.028.8123131
CGCTCTG16950.028.4583325
TTACACG3204.5474735E-1127.0005424
CGTTTCG1607.165295E-526.9982519
GTGTTAG17450.024.3542461
GTATTAC6550.024.1935441
TTGCTCT34350.024.1052884
GTGTAAC9450.023.6291451
GTCTCAC32950.023.6094821
GTGTTAA11350.023.48141
GTTTCAC43850.023.3163173
TTCACCG9350.023.100115
Produced by FastQC (version 0.11.5)