FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2596551
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.039.7008258
GTCTTGC25400.034.6019021
CTGTCGC12700.033.4480829
GTCTCGC10750.030.826431
GTACTAA6500.028.8160111
TCGCTCT13250.028.8004724
GTGTTAC12100.028.5778641
GTATTAG9600.027.015011
TTGCTCT34100.026.8156914
GTATTAT8600.025.9679161
GTCTCAC30150.024.6106871
GGTATAC4700.024.5242651
GTGTACG2951.5303158E-824.420351
TAACCCG2401.6990616E-623.9999335
GTTTCAC39550.023.6666343
TCCGTAC1851.9195022E-423.3517363
GTATTAA8200.022.841961
TCTCGCT14200.022.8216722
GTGTTAG17200.022.6172181
CGCTCTG14450.022.4220815
Produced by FastQC (version 0.11.5)