FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361714
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10500.042.5135088
GTCTCGC10400.035.4248051
GTCTTAC10150.033.450491
GTATTAG9150.033.1588021
CTGTCGC12800.032.62449
TCTCGCT11650.030.3205812
GTATAGC5700.029.1489961
GTATTAT7200.029.096191
TCGCTCT12100.028.5614574
GTCTTGC23300.028.2134421
CTCGCTC12900.026.8208713
TCCGTAC1701.0733155E-425.4403863
GTACTAT5400.025.417361
GTATAGA7050.024.5919381
GTATAAG7650.024.5517541
GGTATAC5600.024.5095961
GCGTTAT1500.001526620924.0796051
GTGTAAC7350.023.5881841
CGCTCTG13500.023.4662365
GGGTATA8750.023.116421
Produced by FastQC (version 0.11.5)