FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486863
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10450.046.8474468
GTCTCGC10500.043.3670041
CTGTCGC12750.040.09059
TCGCTCT11850.037.667494
CTCGCTC13100.033.5628363
TCTCGCT13750.033.0399252
GTCTTGC22700.030.885461
CGCTCTG13700.028.9025025
GGTATAC4650.027.9787161
GTGTTAG16850.027.8818551
GTATAAT6650.027.172311
TTACACT13650.026.3712464
GTGTAAT10800.026.1005131
TACGCTC1751.3177056E-424.6834875
GTATTAA8550.024.5154631
AATACCG2101.643087E-523.9978355
GTCTCAC30200.023.9332281
ACTTCGC1851.9208578E-423.3492438
GTGTTAC9600.023.3398841
GTACTAC4053.274181E-1123.2004571
Produced by FastQC (version 0.11.5)