FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486863
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10450.051.6729668
GTCTCGC11900.039.8928031
CTGTCGC13750.038.7478379
GTCTTGC23000.033.659381
TCGCTCT14050.033.3085334
TCTCGCT15750.027.4414772
CGCTCTG15000.026.3991435
GTATAGG5300.026.1821821
CTCGCTC15750.024.6834243
GTATTAC6100.023.9457441
GTGTTAG14800.023.6868691
GTATAAG7450.023.5278971
TACACCG2452.033279E-623.5094435
TTATACT7400.023.3505954
GTACAAT8450.023.3364731
GTGTAAA16300.023.2993551
GTCTTAC9950.022.7544731
GTATAGC5800.022.6658821
GTATTAT8300.021.9983481
GTACAGA14100.021.7549631
Produced by FastQC (version 0.11.5)