FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164019
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7950.035.3188678
GGTATAC6050.032.24751
TAGTACG1204.2283037E-429.999094
CTGTCGC10550.027.9795489
GTATTGC6750.027.8328231
GTCTCAC26250.026.9764291
GTGTTAG15600.025.9388751
GTGTTAC9000.024.8889681
GTATTAG8150.024.8249361
TTATACT7450.024.1603414
GTCTCGC8400.024.0860961
TATCCGG1500.001556784523.9987165
GTATTAT7050.023.5736271
GATCCGT2151.9705381E-523.4406076
TAATACG1851.9199666E-423.3506414
GTTATAC4557.2759576E-1222.175723
GTCCGGA2954.005451E-722.0449031
GTCTTGC21000.022.0215761
GTATAGA6650.021.7318171
GTATAGT6700.021.5696371
Produced by FastQC (version 0.11.5)