FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164019
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7950.046.1878558
CTGTCGC11550.033.661749
GTCTTGC22150.031.6428621
TCGCTCT9550.030.9097294
GTATTAG7400.030.5849821
GTCTCGC9500.029.9722441
GTACCGA1306.225263E-428.0805051
TACACCG2105.3029544E-727.4280825
TCTCGCT10500.026.0663152
CGCTCTG12100.025.586325
GTATAGG6650.025.251341
GGCGAAC1751.1980535E-425.0317651
CTCGCTC11250.024.9578253
GTGTATA13800.023.8073861
GTATTGT8300.023.750021
TTGCTCT25900.023.3503954
GCATACG2202.1128213E-523.2302381
GTCTTAC8250.023.0089951
GTATTGC6350.022.9950621
CTGATCG2202.3532477E-522.9086829
Produced by FastQC (version 0.11.5)