FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178880
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC907.188758E-540.5549661
GTCTCGC9850.038.5375141
GTATTAC5000.032.1195341
GTCTTGC20250.031.7229941
TCTGTCG8900.029.9300028
TGTACGC2105.2912765E-727.4350222
CCGTGTT8450.027.2639229
TCGCTCT12500.027.0696664
TCTCGCT13850.026.5188672
CTGTCGC10600.026.488289
CTCGCTC13750.025.1323783
GTACGCC1450.001278050824.8254453
GTGTTAC9600.024.3329811
GTATTAG7350.023.8363881
GGTATAC4950.023.5956151
GCGCTAT1550.001738393623.5480441
CGCTCTG14150.023.4043435
GTGTACG3452.743036E-923.2750261
GTCGTAG1902.0947192E-423.0522981
ACCGTGT10450.022.7348828
Produced by FastQC (version 0.11.5)