FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927520
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8450.044.4660841
TCGCTCT9600.038.99934
TATTACG750.001524647538.453182
TCTCGCT10600.036.0498542
TCTGTCG7800.035.9984178
CTCGCTC10950.035.5458643
TAACGGT850.002837866833.8808639
GATAACG950.00485815730.3577732
CTGTCGC9350.030.0307679
GTATTAT6300.029.8205071
CGCTCTG11100.029.1879065
GTGTAAG12300.028.1980061
GTCTTGC19750.026.7077941
GTACTAT5250.026.1502931
GTACTAC3357.8216544E-1125.8832441
GTTTCAC29750.025.1974033
GTGTTAT8150.024.8246251
GGGTAGT6200.024.4742771
TTCACCG5150.023.7659445
GTACTAG4600.023.5621911
Produced by FastQC (version 0.11.5)