FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927520
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8300.045.9716578
GTCTCGC9100.035.2655561
CTGTCGC10600.033.9591269
GTCTTGC17950.032.5061041
TCATACG950.004893361630.3129884
GTCTTAC7400.029.5684761
TCGCTCT10000.028.7973384
GTATAGG6300.027.784981
CTCGCTC11050.026.7124633
GCGTTAT1100.00939537126.5220281
GTCTCAC24250.026.1665761
TCTCGCT11850.026.1350152
GTATTAT7300.025.9770531
GGTATAC3657.2759576E-1225.9770531
GTATAAG5650.025.8179041
CGCTCTG11350.025.3721055
GTATTAA4900.025.304181
GTATAGT6400.025.0716041
GGCGTAC1751.2062362E-425.0064811
GTACTAG4550.024.0446971
Produced by FastQC (version 0.11.5)