FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992655
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.034.8909238
TCGCTCT14800.032.1068344
GTCTCGC12950.030.5866241
CGGATCG1000.006286517728.7988594
GTCTTGC29050.028.261641
GTATTAC8600.027.6346631
CTCGCTC17000.025.8342693
GTACCGG3109.295036E-1025.5546321
TAACACT14500.025.3231324
CTGTCGC18800.025.275599
TCTCGCT16500.024.4353942
CGCTCTG18000.024.3990335
GTGTTAG20500.023.1861521
GTATAAC6900.022.9621331
TAATACT8900.022.6507874
GTATAGA9300.022.45711
GTATAAT9650.022.3888911
GTATTAG12150.021.9312841
GTATAGC8550.021.9000871
GTGTACG5350.021.5379731
Produced by FastQC (version 0.11.5)