FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881956
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7100.051.713218
GTCTCGC7500.039.4956931
CTGTCGC9350.037.72899
GTCTTGC18000.036.5254861
GTATTAC5000.030.3442541
TCTCGCT8850.030.1403712
TCGCTCT8700.029.7909564
GTATAAT5900.029.3891071
CCGATCG1000.006287740528.7971639
GTATATA8650.027.5629041
GTATAGT5800.027.40451
AACCGTA1358.423711E-426.664047
CTCGCTC9850.026.3408243
CGCTCTG10700.026.2404025
CTTGCTC23150.025.839863
TTGCTCT24550.025.8066984
TAATACT5900.025.6252754
CGTCCGT1701.0821663E-425.409266
GTATAGA6000.025.2868791
GTGTAAA11750.025.2100161
Produced by FastQC (version 0.11.5)