FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881956
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7550.042.9097758
GTCTCGC8550.040.1360781
TCGCTCT9550.033.921634
CTGTCGC9650.033.5718969
CGCTCTG9500.033.344165
GGTATAC3800.032.6639331
GTCTTGC19200.032.3236851
GTATAGA5000.030.665671
GTATTAA6550.030.0971681
TCTCGCT11350.029.831922
GTACCGT1254.94688E-429.20541
GTGTAGC9150.027.9286631
GTATTGA5650.027.137761
GTGTAAC6250.026.8689671
CTCGCTC11550.026.8011883
GTCTTAC8900.026.2520451
CGTAGAC1400.001041890625.7104433
GTGTAAA11200.025.4243451
GTATTAG6400.025.098391
GGGTACG2051.222315E-524.9314381
Produced by FastQC (version 0.11.5)