FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952376
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7350.046.0367248
GTCTCGC8650.043.4250831
TCGCTCT10250.039.3340954
CTCGCTC10550.036.2035643
TCTCGCT11400.032.8897252
CGCTCTG10600.032.6008765
GTCTTGC18200.032.5458681
CTGTCGC11250.030.7172689
TAATACT5200.029.075084
CCCGATT1856.1457467E-627.240828
GTGTCGC2207.7344885E-726.2676181
GTATTAC5000.026.0049421
GTATTAT6400.025.9597951
GTATACG1400.001021313525.7985531
GTATATA9300.024.8553811
ACCGTGT6650.023.817438
GGTATAC3952.3646862E-1123.7738571
GTTAGCC10400.023.55993
TTCACCG6500.023.259475
CTAACCG1550.001884389323.2237429
Produced by FastQC (version 0.11.5)