FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952376
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8350.034.960111
TCTGTCG8150.034.4503258
GTCGTAC850.00265543734.3431661
CTAGTCG850.002838743633.878754
GTCGTAG1301.5825814E-533.682721
GTCGAAC1153.0432863E-431.7300971
TCGCTCT10100.029.2246134
CTGTCGC9400.029.1032879
GTCTTGC17500.028.7746681
CGCTCTG9750.028.0585545
GTATTCG1855.5292094E-627.6137621
TCTCGCT11400.025.9032612
CTCGCTC11300.024.8462773
GTCCGTT1751.3177084E-424.6830887
GGTATAC4750.024.582481
TAGTACT5000.024.4773984
TCGCACT1801.5952578E-423.9974484
GTCCAGA14000.023.978891
TTGCTCT25600.021.6539484
GTATTAT4801.2732926E-1121.2856081
Produced by FastQC (version 0.11.5)