FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103491
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13050.030.9033051
GTCTTGC30550.029.4663411
TCTGTCG11700.028.9224078
TCTCGCT15000.026.8858742
TCGCTCT15300.025.8880124
GTCTTAC13600.024.3582631
AGTCGAC1801.5946533E-423.9994457
GTATAGG9350.023.8768751
GTGTAAC9800.023.515341
GGTATAC6550.023.0890141
GTGTAAA19950.022.7418121
TTGCTCT39500.022.6074714
CTCGCTC16950.022.5181943
GTATTAA10400.022.158691
TATACTG9450.022.0947275
CGCTCTG16700.021.9875165
GTATTAT10000.021.6047211
GTGTAAT12350.021.5755651
TACACTT10400.021.461045
GTGTTAG22200.021.4100841
Produced by FastQC (version 0.11.5)