FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103491
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13550.040.92541
TCTGTCG13850.038.469678
TCGCTCT14250.037.389824
CGCTCTG15800.030.9876295
CTCGCTC16450.030.638583
GTCTTGC28500.030.0707041
TCTCGCT17500.029.212172
GTATTAG10050.028.6639081
GTATAAC5650.028.0424371
GTGTTAC11000.027.4978081
GTATATA11650.026.581781
GTGTAAC9950.026.0567931
GTCTTAC11650.025.9635981
GGTATAC7600.025.5853671
GTATAAG8300.025.1629411
GTATTAA10400.024.9293351
CTGTCGC22950.024.1570879
GTATTGG11000.023.5695511
GTATTAC8400.023.1486661
GTGTTAG21950.022.967041
Produced by FastQC (version 0.11.5)