FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692731
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.034.868868
GTCTTGC26850.034.863121
GTCTCGC12350.032.067341
GCGTAAC1352.2461716E-532.002561
GTGTAAC9000.027.2021731
TCGCTCT13850.027.0346534
GTATAAT9500.026.5284371
TTGCTCT38800.025.6102944
TAATACT9000.025.6020474
GTGTTAC13650.025.3207071
GTATAAC5800.024.829571
TCTCGCT15150.024.7148482
CTCGCTC14300.024.67333
CTGTCGC16050.024.2235379
GTATTAA10100.023.5266341
GTACCGC1851.9201504E-423.3506166
GTACGGC1550.001882724223.2276611
GTGTAAA18950.022.7986561
CTTGCTC37650.022.7587933
CGCTCTG14950.022.634745
Produced by FastQC (version 0.11.5)