FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692731
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11100.038.2828981
TCTGTCG12350.037.894578
GTATTAT9500.031.8420831
CTGTCGC17550.029.5383329
TCGCTCT14100.029.1062564
GTATTAG9800.027.9275781
GTCTTGC27550.027.71151
CTCGCTC14800.026.2701553
TCTCGCT14600.026.1368712
CGCTCTG15400.024.7791125
TACCGGA1751.3169782E-424.6856062
GTATAAT9950.024.6111321
GTGTTAC12900.024.0079211
GTATCGC1801.5907628E-424.0079211
TAATACT9150.023.6064554
GTATTAC8600.023.4495961
GTACCGG2852.964989E-722.7443431
GTGTTAG20100.022.5746121
GCGTAAT1952.7356224E-422.1611561
TTGCTCT36250.022.04684
Produced by FastQC (version 0.11.5)