FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839798
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.047.3665168
CTGTCGC17100.033.6828549
GTATTAC8450.028.1251351
TCGCTCT13900.027.9779824
GTCTCGC13200.027.8249021
GTGTTAC13650.025.8523981
CTCGCTC15150.025.6695673
TATACTG10450.024.802835
TCTCGCT15100.023.8468172
GTGTAGG19350.023.075341
GTATTAG11550.023.0705071
CGCTCTG16650.022.9179975
GTCTTGC29900.022.6409191
GTATAAC6400.022.5054341
GTATTAT10550.021.844141
TACACTG18800.021.8289095
GTCTCAC34350.021.5947341
GGATCGT3707.219569E-921.4045438
TTACACT17650.021.2175874
GTGTTAG22450.020.851361
Produced by FastQC (version 0.11.5)