FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839798
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12400.030.2017771
GTCTTGC29200.030.0903911
TCTGTCG14650.028.5053588
TCGCTCT13750.027.752964
GTATTAT10950.024.993111
GTGTTAG20050.024.0664081
CTGTCGC19000.023.4949329
GTCTCAC34650.023.279071
GTGTAAA20400.022.594391
GTATAAG8300.022.5603641
GTATTAA10600.022.4212021
GTGTTAC14500.022.3509161
GGATCGT4850.022.2682278
CTCGCTC14650.022.1154463
GATCGTT4900.022.0409989
GTATAGA10650.021.6396981
GTATAAC5700.021.4795361
ATAGTAC7450.021.2611753
GTCTTAC13450.020.8830131
TAGTACT7600.020.842284
Produced by FastQC (version 0.11.5)