FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238242
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC4950.037.8253021
TCTGTCG10400.035.9987348
GTCTCGC10500.033.6063231
TCGCTCT10700.030.2860744
CGTTTCG1255.370057E-428.7989889
GTATAAC5050.028.520221
CTCGCTC11400.028.4264033
TCTCGCT12350.027.9890732
GTGTTAG16350.027.748341
GTCTTGC21600.027.6718751
CGCTCTG12700.027.2116415
GTATAGA7700.027.1219861
GTGTAAC7850.026.6037311
GTATTAC5850.025.8510191
TAATACT7650.025.4165484
GTATAAG8500.025.4165481
GTGTAAG11800.024.4113731
GGGTAAC8600.023.4462741
TTGCTCT29400.023.2696864
ATAATAC8200.022.8335673
Produced by FastQC (version 0.11.5)