FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238242
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10200.042.3700261
TCTGTCG10050.039.404798
TCGCTCT12250.037.618054
TCTCGCT12100.036.299182
CTCGCTC12850.034.1790243
GTATTAA7800.030.4738241
GTCTTGC22100.030.3108631
CTGTCGC14750.029.2894749
CGCTCTG14600.029.0972235
GTACTAG5850.025.856581
GTATAAG7650.025.4220161
GTATTGG8250.025.31931
GTACTAC5700.025.2733481
TACACTG16100.024.5973995
CGTTACT1801.5937144E-424.0010992
GTATAGG6400.023.634531
GTATAAT8250.023.5731411
GCGTTAT1851.9143618E-423.360771
TAATACT8050.023.2557224
GTCTTAC9350.022.3405591
Produced by FastQC (version 0.11.5)