FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628856
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10150.038.303588
GTCTCGC12550.033.8585741
CTGTCGC14300.030.7118139
GTATTAC5900.030.517461
GTCTTGC25550.030.1615221
TCGCTCT13200.029.4632194
GTATTAG9100.029.2833461
GTCTTAC11600.027.9392591
TCTCGCT14250.027.7976592
TATTACG1100.00999190426.189534
CTCGCTC15300.024.948523
CGCTCTG15450.024.6978455
GTATTGC7250.023.8415031
GTATAGC5950.022.9983671
GTATAAT7900.022.7915211
TTGCTCT34400.022.611314
GATCGTT3203.6507117E-822.498929
GTGTTAG19250.022.4481681
ATAATAC8500.022.0300163
GGGTTAC8600.021.7738531
Produced by FastQC (version 0.11.5)