FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737141
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.033.961138
GTCTCGC11300.033.141321
GTCTTGC26500.032.3404461
TCGCTCT12650.029.0351814
TCTCGCT13450.027.3081842
GTATAGG8000.027.006991
CTCGCTC13400.026.3351753
CTGTCGC16550.026.1018479
GTATTAG10400.024.929531
GTATCGC1450.001275566424.8340131
GTATTAT10200.024.0062121
TTGCTCT36650.023.776964
CGCTCTG13250.023.3652555
GGATAAT13700.023.1300741
GTGTTAC12400.022.6510241
GTACCGT1600.002261998422.5058251
GTGTAGA15700.022.4771541
GTATATA15750.022.4057981
GTCTTAC11650.022.2546861
GTGTAGC14350.022.082371
Produced by FastQC (version 0.11.5)