FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737141
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.039.271628
GTCTCGC12850.039.2300071
TCGCTCT13900.037.293924
GTCTTGC25700.032.5048641
CGCTCTG15600.030.9222095
TAATACT9600.030.7491384
CTCGCTC17050.029.9815653
TCTCGCT17400.027.7243732
CTGTCGC18600.027.0960149
GTCCGGT2456.8803274E-826.4545211
TAAACCG2207.962426E-726.1810845
GTATTAC6350.026.0842021
GTACTAG6450.025.6797981
GTGTAAG14700.025.4747241
GTCTCAC34550.024.59561
GTATAGA8300.023.4265921
GTATTAG10800.023.338221
GTGTAAC10850.023.2306731
GGTATAC5650.022.9428581
GTGTAAA19950.022.7416061
Produced by FastQC (version 0.11.5)