FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926241
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13550.040.925861
TCTGTCG11300.040.7783058
TCGCTCT14850.036.8480764
CTGTCGC12900.035.16249
TCTCGCT16000.033.3087352
CGCTCTG17100.032.841745
CTCGCTC17050.030.4074693
GTCTTGC27550.028.7552721
GTATAAT9750.027.3302441
ACCGTGT9500.025.7681338
GTCGTAA1751.3146343E-424.692191
GTGTTAG21900.024.6640011
GTATTAG12050.024.504351
GTGTTAC12900.024.0062961
TTATACT10350.023.6519094
GCGTTAC1851.9163881E-423.3574771
TAATACT10300.023.0677034
GTACTAG6000.022.805981
GTATACG1902.2957691E-422.7428051
GGAGTGC65900.022.7250885
Produced by FastQC (version 0.11.5)