FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868429
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12350.043.7231528
GTCTCGC11300.041.426021
TCGCTCT13450.035.3394664
CTGTCGC18050.032.3091059
TCTCGCT14400.031.5076732
GTCTTGC28900.030.9002591
CTCGCTC15600.030.0073073
CGCTCTG16050.029.1578485
GTGTAAC9450.028.1973421
GGTATAC6450.027.9137761
GTATTAG12450.027.7657991
GTGTAAT12500.026.5024551
GTCTCAC36150.024.902331
GTGCGAA3001.8355422E-824.0058481
GTGTAAA20250.023.4723831
GTATTAC7700.023.3823181
ATACCCG2802.5018926E-723.1484954
GTATAGA9850.022.6654191
GTCTTAC11500.022.5446221
GTACGAA1600.002262226322.5054821
Produced by FastQC (version 0.11.5)