FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868429
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12600.038.2867778
GTCTCGC11850.035.247631
GTCTTGC26650.034.0481571
TCGCTCT13800.031.305224
CTCGCTC15050.027.2693883
CGCTCTG14850.025.6976835
TCTCGCT16600.025.1568892
GGTATAC7750.025.0889431
TACTGCG3451.1823431E-1025.0441747
GTGTTAC13650.024.7962231
CTGTCGC18950.024.697269
GTATAAT9350.024.6466941
GTATAGG9100.024.5324331
TTGCTCT37900.023.1774274
ACCGTGT10100.022.8125178
GTATAGA8850.022.784271
GTACTAC7300.022.6895181
TATACTG10950.022.3567875
GTTTCAC46350.021.5924593
TTACACT14700.021.551624
Produced by FastQC (version 0.11.5)