FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3007919
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14100.042.394231
TCGCTCT15850.037.702824
TCTGTCG13200.036.5448278
CGCTCTG16300.035.3368155
GTCTTGC28550.030.5229871
TCTCGCT18700.030.0320682
CTCGCTC19850.027.2035623
CTGTCGC19850.025.7527079
GTGTTAG22400.025.0780851
GTATTAG10950.024.9929331
GTATTAC7700.024.3181441
GTATAGT10000.023.7663081
GTGTTAC13050.023.7304361
GGGTTTC51800.023.4965841
GTATTAA9300.023.2319721
GTCTTAC12700.022.6831861
TAGTACT9350.022.3311674
GTGTAAC10400.022.1597291
GTTCGGA1952.736919E-422.1597271
TTCACCG8900.021.8423215
Produced by FastQC (version 0.11.5)