FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054981
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15800.041.474121
TCTGTCG13750.040.319018
CTGTCGC16500.037.0899969
TCGCTCT16300.036.2199674
TCTCGCT19200.032.629542
GTCTTGC30550.032.528261
CTCGCTC18800.031.7885343
CGCTCTG17050.029.9816685
GTGTTAG20700.027.134211
GTATTAG12150.026.6703761
GTATAAT10000.026.6437051
GTATTAC7250.025.8242821
GTGTTAC13850.025.4764691
GTGTAAT13900.023.8306541
GTCTCAC36200.023.67181
GTCTTAC11500.023.168441
GGGTTTC53150.022.7613981
GTACATA9250.022.5761131
TTGCTCT39950.022.3473874
TTACACT17750.022.3093134
Produced by FastQC (version 0.11.5)