FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054981
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13650.036.3942878
GTCTTGC27800.033.678321
GTCTCGC13000.032.131911
ACCGTGT9900.030.544358
TTCACCG9500.029.5568245
TCGCTCT13700.029.4295944
CTGTCGC19300.026.1143289
CTCGCTC15250.025.9662743
GTCTCAC36700.025.7073381
CCGTGTT10450.024.8041489
GTACTAC4700.024.5173761
TCTCGCT16600.024.2958342
GTGTTAG20300.024.1248571
CTCGATC11750.023.2842668
GGTATAC5900.023.1928161
TTGCTCT39750.023.1840694
CGCTCTG15600.023.076095
GGGTTTC53600.022.9764651
GTATTAG11450.022.6437781
GTTTCAC47900.022.395853
Produced by FastQC (version 0.11.5)