FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091603
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12950.042.26321
TCTGTCG13600.038.1171848
TCGCTCT15000.036.4795574
CGCTCTG16600.030.7948075
CTGTCGC18050.030.7148049
GTCTTGC30250.030.7102151
TCTCGCT17450.029.3009342
CTCGCTC18400.028.564873
GCGTAAT1306.746023E-427.6977941
GTGTTAG21400.027.2577381
TTGCTCT40850.026.614124
GTGTAAC10050.025.7961561
GTATTAG11800.023.1910341
GTCTCAC36750.023.1229481
GTCTTAC13450.023.023151
GTGTAAT12250.022.926991
CTTGCTC39100.022.8334853
GTCCGGA4505.456968E-1222.404441
TCTGTCA41700.022.2731118
GGGTAAT11400.022.1096421
Produced by FastQC (version 0.11.5)