FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3192539
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13250.043.478211
TCTGTCG13300.040.0598758
TCGCTCT15200.037.4207954
CTGTCGC16500.033.1634069
TCTCGCT17150.032.331372
CTCGCTC16650.029.8381023
GTCTTGC30300.028.7567831
GTGTTAC14150.027.481151
CGCTCTG17750.027.1772775
GTACTAG6750.026.6706621
GTATTAT10550.026.62011
GTTACCC11850.024.9116123
GTACTAC6150.024.5890481
GGTATAC6200.024.390751
GTATAAG9600.024.0035951
GTGTAAA20750.023.9457551
GTTTCAC48600.023.8520623
GTATTAC8550.023.582481
GTACTAT7550.022.8908461
GTATAAT10150.022.7029081
Produced by FastQC (version 0.11.5)