FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088158
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.038.0984158
GTCTCGC11850.037.0672571
TCGCTCT13950.033.0320474
CTGTCGC14150.031.5475069
GTCTTGC28300.027.9888631
TCTCGCT15450.027.9637052
GTATTAC7250.027.8098741
CTCGCTC15900.027.1700823
CGCTCTG16500.027.0543755
TAATACT11350.026.0086484
GGGTACG2851.0657459E-825.2658621
ACCGTGT10000.023.759858
GTATAAT9950.023.1582581
GTGTTAC12750.023.1554181
GTATTAG11250.023.0424671
TTCACCG10950.023.0135545
GTCTCAC36750.022.7289621
GTATTAT11150.022.6033171
GTGTAGA15250.022.192541
GTTTCAC48450.022.1426333
Produced by FastQC (version 0.11.5)