FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013542
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC5150.033.5609741
TCTGTCG12400.031.9352748
GTCTTGC27500.031.4252781
CTGTCGC14150.030.0210029
GTCTCGC12750.028.2416691
GTATACG2456.879782E-826.4549521
GTATTAT10400.026.313631
TCGCTCT14550.025.2369484
GTATTAG11500.025.0491311
GTATAGA9000.024.80561
GTACTGA11550.024.3171791
GGATACG2401.6958365E-624.0054191
GTGTAAA18550.023.2936671
CTCGCTC16150.022.7370683
GTCTCAC34500.022.544221
GTGTTAG20000.022.3250391
GTATAGT9400.022.2177831
GTATTAA10700.022.210621
GTGTAAG15900.022.1936871
TCTCGCT16050.021.986272
Produced by FastQC (version 0.11.5)